Carnoy’s Fluid is a widely used fixative in histology and cytology. Composed primarily of ethanol, chloroform, and acetic acid, it provides rapid fixation and excellent preservation of cellular morphology, making it particularly effective for chromosomal analysis and tissue sample preparation. Carnoy’s Fluid is known for its ability to preserve delicate structures and is also used for DNA isolation in certain applications.
Applications of “Carnoy’s Fluid”
- Histology and Cytology:
- Carnoy’s Fluid is frequently used in histological procedures to fix tissue samples quickly, maintaining cell structure and ensuring the integrity of the samples for microscopic analysis. It’s particularly beneficial in preserving nuclear details, making it ideal for chromosomal studies.
- DNA and RNA Preservation:
- Due to its alcohol-based composition, Carnoy’s Fluid is useful in the preservation of nucleic acids. It helps maintain the structure of DNA and RNA during fixation, making it an essential reagent in molecular biology for DNA isolation and RNA studies.
- Cancer Research:
- In cancer diagnostics and research, Carnoy’s Fluid plays a critical role in preparing cytological smears and tissue sections for the detection of cancerous cells, as it fixes samples rapidly without causing significant tissue shrinkage or distortion.
- Microbiological Studies:
- Carnoy’s Fluid is used in microbiology to study cell division and other processes, as it allows for precise chromosomal staining without damaging the cell’s finer details.
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